App ID: FLM-C220017

Comixsil HCS利用陽離子交換與氫鍵作用分離核苷（MS匹配)

1. Thymidine(胸苷)

2. Uridine(尿苷)

3. Deoxyadenosine(脫氧腺苷)

4. Adenosine(腺苷)

5. Deoxyguanosine(脫氧鳥苷)

6. Guanosine(鳥苷)

Column: Comixsil HCS 3u 100*3.2mm (P/N: FMD-HCS3-RONU)

Mobile phase of channel C preparation:

Place 50 ml of formic acid in 1 L volumetric flask. Add 800 ml of ACN and stir. Add 5 g of ammonium formate and stir until all ammonium formate is dissolved. Dilute to 1 L with ACN. This will create 5% formic acid and 0.5% ammonium formate in ACN to be used in channel C.

Q-Pump set up:

A: ACN: B: MeOH C: 5% formic acid with 0.5% ammonium formate in ACN

Gradient set up:

Time min A% B,% C%

0        88        10         2

8        48        50         2

8.01    88       10          2

Run time 16 minutes

Flow rate: 0.8 ml/min

Detection: 275nm

本應(yīng)用適用于核苷在質(zhì)譜檢測器下的分析